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PDPK1 Antikörper (AA 457-556)

Name: PDPK1 Antikörper (AA 457-556)
Brand: antibodies-online
SKU: ABIN5542259
Price: 657.84 EUR
Availability: InStock

Dieser Maus Monoklonal Antikörper erkennt spezifisch PDPK1 in WB, IHC, ELISA, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Affe.

Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (PDPK1 Antikörper (AA 457-556))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PDPK1 Antikörper (AA 457-556))

ELISA

Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate. (PDPK1 Antikörper (AA 457-556))

Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate. (PDPK1 Antikörper (AA 457-556))

Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red). (PDPK1 Antikörper (AA 457-556))

FACS

Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). (PDPK1 Antikörper (AA 457-556))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining. (PDPK1 Antikörper (AA 457-556))

IHC

7 Abbildungen

Produktnummer ABIN5542259

Kurzübersicht für PDPK1 Antikörper (AA 457-556) (ABIN5542259)

Target

Alle PDPK1 Antikörper anzeigen

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reaktivität

Human, Maus, Ratte, Affe

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser PDPK1 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon

7E4G11

Discover our top product PDPK1 Primärantikörper

Bindungsspezifität
28

23

15

15

15

15

8

6

6

4

4

4

3

3

3

2

2

2

2

2

2

2

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2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 457-556

Verwendungszweck

PDPK1 Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Isotyp

IgG1
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibody
PDPK1 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibody
PDPK1 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibody
PDPK1 Reaktivität: Human WB, PLA Wirt: Maus Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 Reaktivität: Human, Ratte, Affe WB, IHC, ELISA, ICC, FACS Wirt: Maus Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibody
PDPK1 Reaktivität: Human WB, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 277-556) antibody
PDPK1 Reaktivität: Human IHC, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pTyr373), (pTyr376) antibody
PDPK1 Reaktivität: Human, Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung

PDPK1

Hintergrund

Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.

Molekulargewicht

63.2 kDa

UniProt

O15530

Pathways

PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals